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Capan-2 has been reported by several groups to be wild-type for TP53, but it should be noted that Berrozpe found a 200-bp deletion 101. In support of Capan-2 possessing wild-type TP53 is a study showing that radiation was able to induce elevated TP53 and p21WAF1/CIP1 protein expression in Capan-2 cells, suggesting the presence of a …
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In the HPAC and Capan-2 cells with wt-p53 and in the MIA PaCa-2 cells with mt-p53, overexpression of the mesothelin significantly decreased bax levels and increased bcl-2 levels, followed by increased proliferative and colony forming capability. Furthermore, mesothelin-shRNA-transfected cells exhibited a reduced rate of tumor growth under in ...
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CVCL_0026. To cite this cell line use: Capan-2 (RRID:CVCL_0026) Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE). …
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HTB-80 ™. Capan-2 is a cell line with polygonal morphology that was isolated in 1975 from the pancreas of a 56-year-old, White, male patient with pancreatic adenocarcinoma. …
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To investigate the antitumor effect of Gem on Capan-2 Luc cells in vitro, cells were incubated with various concentrations of Gem for 48 h. After drug exposure, cell proliferation was determined by MTT assay. The results showed that Gem could inhibit the proliferation of human pancreatic cancer Capan-2 Luc cells in a dose-dependent manner …
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
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Capan-2 cells were transfected with miR-222 mimics (50 nM), inhibitors (100 nM) or their negative controls for 48 h using Lipofectamine 2000 (Invitrogen, USA) according to the manufacturer's instructions. P57 siRNA was purchased from RiboBio (China). P57 siRNA (100 nM) was transfected into Capan-2 cells with similar method described above.
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